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KMID : 1147220180190040092
Journal of Biomedical and Translational Research
2018 Volume.19 No. 4 p.92 ~ p.102
Screening of immunoactive ingredients in frequently consumed food in Korea
Gil Na-Young

Lee Sang-Myeong
Mun Ji-Young
Yeo Soo-Hwan
Kim So-Young
Abstract
The objectives of this study were to find out the plant to enhance immune activity among 42 kinds of foods frequently consumed by the Korean elderly consisting of 5 food groups and 5 wild plants. Each sample was assessed the immunoactive effect by measuring NF-kB/AP1 gene expression, nitric oxide and cytokine production in RAW-Blue¢â cell. Soybean sprouts of 47 plants showed the highest NF-¥êB/AP1 gene expression at the level of 1.13 ¡¾ 0.03 (O.D. 650 nm) and Soritae, sweet potato, banana, apple, garlic, crown daisy, cabbage and Ailanthus altissima also had high activity of NF-kB/AP1 gene in RAW-Blue¢â cell stimulated by LPS. NO production of Ailanthus altissima was significantly higher than that of other plants and 16 plants of glutinous sorghum, black rice, Seoritae, Heuktae, sweet potato, banana, apple, garlic, mungbean sprouts, spinach, crown daisy, young pumpkin, cabbage, soybean sprouts, Actinidia arguta and Aster scaber were the next best activity. The above results selected 17 out of 47 plant samples. Moreover, soybean sprouts was significantly shown to increase TNF-¥á (1,509.55 ¡¾ 1.38 pg/mL) and IL-1¥â (54.56 ¡¾ 1.08 pg/mL) cytokines in comparison with RAW-Blue cell stimulated by LPS. According to the results of in vitro evaluation, the ethanol extract of soybean sprout increased the production of immune-enhancing cytokines by proliferation of macrophages. In addition, NF-kB transcription factor activity and NO production ability were excellent, and it was selected as a material having excellent immunological activity.
KEYWORD
immunoactive effect, frequently consumed food, ethanol extract, NF-kB, cytokines
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